Validating transcripts with probes and imaging technology


09-Jul-2019 15:18

In many epithelial tissues, such as the skin and the intestine, there is a clear hierarchical partition into stem cells and diverse differentiated epithelial progenies, each of which displays distinct phenotypic and morphological features.

The precise location of cells within the tissue translates to constant changes in the levels of niche-secreted morphogens, which give rise to position-modulated gene expression programs.

Such measurements allow one to infer the function of genes based on their co-expression patterns.

However, the variability in single cell gene expression in most biological systems and especially in tissues and tumors suggests that bulk transcriptome measurements should be complemented by techniques aimed at characterizing gene expression programs in individual cells Bulk transcriptome measurements inform on the average gene expression in a sample.

High throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples.

However, the analysis of spatial gene expression patterns with single cell resolution requires quantitative hybridization (FISH) techniques that facilitate detection of individual fluorescently labeled m RNA molecules of practically any endogenous gene.

Most solid tumors consist of a mixture of cancer and stromal cells.

Here we present click-amplifying FISH (clamp FISH), a method for fluorescence detection of nucleic acids that achieves high specificity and high-gain (400-fold) signal amplification.These methods, which are based on advances in probe design, imaging technology and image processing, enable the absolute measurement of transcript abundance in individual cells with single-molecule resolution.



I am I am an optimistic, active, creative and romantic woman..… continue reading »


Read more

You can also expect that I am going to be brutally honest in my reviews.… continue reading »


Read more

Free Chat Now's chat room selection is separated by sexuality and interest. All of our chat rooms are intended for adults and the Sex Chat room contains explicit content.… continue reading »


Read more

Have a great chat for i Pad, chat for i Phone, etc.… continue reading »


Read more

Some addresses or other data might no longer be current.… continue reading »


Read more

Bringing you only the hottest sexy cam amateur girls online today.… continue reading »


Read more